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phosphor creb  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphor creb
    Phosphor Creb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphor-creb+antibody/pmc12647362-50-13-0
    Average 86 stars, based on 1 article reviews
    phosphor creb - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Phospho-proteomics:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    Western Blot:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    MTT Assay:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    Control:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    Blocking Assay:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    Incubation:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).

    Negative Control:

    Article Title: Heparin-Functionalized Polymer Graft Surface Eluting MK2 Inhibitory Peptide to Improve Hemocompatibility and Anti-Neointimal Activity
    Article Snippet: The antibodies for CREB and phosphor-CREB were obtained from Cell signaling Technology (Danvers, MA, USA).

    Article Title: Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages.
    Article Snippet: Primary antibodies were pre-diluted in blocking buffer to 1:200 for F4/80 (Ebioscience or Bio-Rad), CD68 (Santa Cruz Biotechnology), VEGFA (Santa Cruz Biotechnology), ARG1 (Cell Signaling Technology), IL-10 (R&D Systems), or phosphor-CREB (Cell Signaling Technology) and applied to tissue sections overnight at 4 C in a humidified chamber.

    Article Title: Scrophularia buergeriana Extract (Brainon) Improves Scopolamine-Induced Neuronal Impairment and Cholinergic Dysfunction in Mice through CREB-BDNF Signaling Pathway
    Article Snippet: For blocking, the membranes were kept for 1 h at 23 ◦C then incubated with the first antibodies for SOD-1, SOD-2, GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax, Bcl-2, cleaved PARP, cleaved caspase-9, phosphor-CREB, CREB (Cell Signaling Technology, Inc., Danvers, MA, USA), and BDNF (Abcam, Cambridge, UK) at a 1:1000 dilution at 23 ◦C for 1 h. The incubated membranes were washed and further reacted with second antibodies (1:1000; GenDEPOT, Barker, TX, USA) at 23 ◦C for 1 h. The membranes were processed for detection using ECL solution (Atto, Tokyo, Japan) and the band intensity was measured utilizing Image-Pro Plus (Media Cybernetics, Inc., Rockville, MD, USA).

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity
    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Article Title: Activation of GLP-1 Receptor Enhances Neuronal Base Excision Repair via PI3K-AKT-Induced Expression of Apurinic/Apyrimidinic Endonuclease 1.
    Article Snippet: The dilution factors for the primary antibodies were as following, APE1 (1:1,000; Santa Cruz), phosphor-AKT (1:1,000; Cell Signaling), phosphor-CREB (1:1,000; Cell Signaling), Ligase III (1:1,000; Santa Cruz), FEN1(1:1,000; Cell Signaling), Pol β (1:200; GeneTex), NEIL1 (1:1,000; Santa Cruz), OGG1 (1:1,000; Novus), UDG (1:1,000; Novus), lamin A/C (1:1,000; Cell Signaling), GLP-1R (1:1,000; Abcam/Alomone Lab) and actin (1:5,000; Sigma-Aldrich).



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    Danaher Inc phosphor creb
    The effect of KG501 (a specific inhibitor <t>p-CREB)</t> on proliferation and apoptosis levels in OGD NSCs. (A) CART55-102(0.8 nM) treatment protects against loss of the structure of <t>NSCs</t> <t>(Nestin,</t> red) and enhances Nestin (Red) expression and KG501 inhibits the protection of structure in cultured oxygen and glucose deprived NSCs. Scale bar = 100 pm. (B) CART55-102(0.8 nM) treatment enhances Sox-2(Green) expression and KG501 inhibits the protection of neural stem cells in cultured oxygen and glucose deprived. Scale bar = 50 μm (C) CART55-102(0.8 nM) treatment increases the proliferation of oxygen and glucose deprived neural stem cells and KG-501 inhibits the proliferation of NSCs in cultured oxygen and glucose deprived. Scale bar = 100um. (D) Quantitative analysis of fluorescence results using ImageJ software. Data was presented as mean ± SEM, *** p < 0.001, ### p < 0.001. (E) The protein levels Bcl-2 and Bax were measured by Western blot in different groups. (F) Quantitative analysis of Bcl-2 expression. Blot images were cropped for comparison. All values are expressed as mean ± SEM of the band intensity of Bcl-2 normalized to Tubulin from 3 independent experiments. Compared with the OGD, after treatment CART55-102 (0.8 nM), the levels of Bcl-2 significantly increased. But the levels of Bcl-2 were significantly decreased after pre-treatment with KG501 in the CART treatment group. ** p < 0.001, *** p < 0.01, ## p < 0.01, ### p < 0.001. (G) Quantitative analysis of Bax expression. Blot images were cropped for comparison. All values are expressed as meant ± SEM of the band intensity of Bax normalized to Tubulin from 3 independent experiments. Compare with OGD, after treatment CART55-102(0.8 nM), the levels of Bax significantly decreased. But the levels of Bax were significantly increased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ### p < 0.001. (H) The protein levels of p-CREB were measured by Western blot in different groups. (I) Quantitative analysis p-CREB expression. Blot images were cropped for comparation. All values are expressed as mean ± SEM of the band intensity of p-CREB normalized to 3 independent experiments. Compare with the OGD, after treatment CART55-102(0.8 nM), the levels of p-CREB significantly increased. But the levels of p-CREB were significantly decreased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ## p < 0.01.
    Phosphor Creb, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphor creb ser133  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc phosphor creb ser133
    The effect of KG501 (a specific inhibitor <t>p-CREB)</t> on proliferation and apoptosis levels in OGD NSCs. (A) CART55-102(0.8 nM) treatment protects against loss of the structure of <t>NSCs</t> <t>(Nestin,</t> red) and enhances Nestin (Red) expression and KG501 inhibits the protection of structure in cultured oxygen and glucose deprived NSCs. Scale bar = 100 pm. (B) CART55-102(0.8 nM) treatment enhances Sox-2(Green) expression and KG501 inhibits the protection of neural stem cells in cultured oxygen and glucose deprived. Scale bar = 50 μm (C) CART55-102(0.8 nM) treatment increases the proliferation of oxygen and glucose deprived neural stem cells and KG-501 inhibits the proliferation of NSCs in cultured oxygen and glucose deprived. Scale bar = 100um. (D) Quantitative analysis of fluorescence results using ImageJ software. Data was presented as mean ± SEM, *** p < 0.001, ### p < 0.001. (E) The protein levels Bcl-2 and Bax were measured by Western blot in different groups. (F) Quantitative analysis of Bcl-2 expression. Blot images were cropped for comparison. All values are expressed as mean ± SEM of the band intensity of Bcl-2 normalized to Tubulin from 3 independent experiments. Compared with the OGD, after treatment CART55-102 (0.8 nM), the levels of Bcl-2 significantly increased. But the levels of Bcl-2 were significantly decreased after pre-treatment with KG501 in the CART treatment group. ** p < 0.001, *** p < 0.01, ## p < 0.01, ### p < 0.001. (G) Quantitative analysis of Bax expression. Blot images were cropped for comparison. All values are expressed as meant ± SEM of the band intensity of Bax normalized to Tubulin from 3 independent experiments. Compare with OGD, after treatment CART55-102(0.8 nM), the levels of Bax significantly decreased. But the levels of Bax were significantly increased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ### p < 0.001. (H) The protein levels of p-CREB were measured by Western blot in different groups. (I) Quantitative analysis p-CREB expression. Blot images were cropped for comparation. All values are expressed as mean ± SEM of the band intensity of p-CREB normalized to 3 independent experiments. Compare with the OGD, after treatment CART55-102(0.8 nM), the levels of p-CREB significantly increased. But the levels of p-CREB were significantly decreased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ## p < 0.01.
    Phosphor Creb Ser133, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p creb phosphor s133 ab32096  (Danaher Inc)
    86
    Danaher Inc p creb phosphor s133 ab32096
    The effect of KG501 (a specific inhibitor <t>p-CREB)</t> on proliferation and apoptosis levels in OGD NSCs. (A) CART55-102(0.8 nM) treatment protects against loss of the structure of <t>NSCs</t> <t>(Nestin,</t> red) and enhances Nestin (Red) expression and KG501 inhibits the protection of structure in cultured oxygen and glucose deprived NSCs. Scale bar = 100 pm. (B) CART55-102(0.8 nM) treatment enhances Sox-2(Green) expression and KG501 inhibits the protection of neural stem cells in cultured oxygen and glucose deprived. Scale bar = 50 μm (C) CART55-102(0.8 nM) treatment increases the proliferation of oxygen and glucose deprived neural stem cells and KG-501 inhibits the proliferation of NSCs in cultured oxygen and glucose deprived. Scale bar = 100um. (D) Quantitative analysis of fluorescence results using ImageJ software. Data was presented as mean ± SEM, *** p < 0.001, ### p < 0.001. (E) The protein levels Bcl-2 and Bax were measured by Western blot in different groups. (F) Quantitative analysis of Bcl-2 expression. Blot images were cropped for comparison. All values are expressed as mean ± SEM of the band intensity of Bcl-2 normalized to Tubulin from 3 independent experiments. Compared with the OGD, after treatment CART55-102 (0.8 nM), the levels of Bcl-2 significantly increased. But the levels of Bcl-2 were significantly decreased after pre-treatment with KG501 in the CART treatment group. ** p < 0.001, *** p < 0.01, ## p < 0.01, ### p < 0.001. (G) Quantitative analysis of Bax expression. Blot images were cropped for comparison. All values are expressed as meant ± SEM of the band intensity of Bax normalized to Tubulin from 3 independent experiments. Compare with OGD, after treatment CART55-102(0.8 nM), the levels of Bax significantly decreased. But the levels of Bax were significantly increased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ### p < 0.001. (H) The protein levels of p-CREB were measured by Western blot in different groups. (I) Quantitative analysis p-CREB expression. Blot images were cropped for comparation. All values are expressed as mean ± SEM of the band intensity of p-CREB normalized to 3 independent experiments. Compare with the OGD, after treatment CART55-102(0.8 nM), the levels of p-CREB significantly increased. But the levels of p-CREB were significantly decreased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ## p < 0.01.
    P Creb Phosphor S133 Ab32096, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    JNK1/2 and p38 signaling affects CREB and STAT3 phosphorylation and subsequent 5-FU-initiated cytotoxicity in Visfatin-stimulated HCT-116 CRC cells. ( A ) HCT-116 CRC cells were pretreated with DMSO (vehicle), SP600125 (a JNK1/2 inhibitor at 20 μM), or SB203580 (a p38 inhibitor at 10 μM) for 30 min and then either maintained as controls or treated with Visfatin (50 ng/mL) for 4 h. The phosphorylation of CREB and STAT3 was determined using Western blot analysis. ( B ) HCT-116 CRC cells were pretreated with DMSO (vehicle), PD98059 (an ERK1/2 inhibitor at 25 μM), SP600125 (a JNK1/2 inhibitor at 20 μM), or SB203580 (a p38 inhibitor at 10 μM) for 30 min and then treated with vehicle (PBS) or Visfatin (50 ng/mL) for 1 h. Subsequently, the cells were then either maintained as controls or treated with 5-FU (10 μM) for 24 h. The viability of the treated cells was examined using the MTT assay. The results in ( A ) were obtained from three independent experiments, all of which exhibited similar outcomes. The data in ( A , B ) were calculated from the results of three independent experiments and are presented as the mean ± SEM. The results were considered statistically significant when p < 0.05. *, compared to untreated control cells. #, compared to cells treated with ( A ) DMSO and Visfatin and ( B ) 5-FU alone. ( B ) **, compared to cells treated with DMSO and 5-FU.

    Journal: Biology

    Article Title: Amphiregulin Upregulation in Visfatin-Stimulated Colorectal Cancer Cells Reduces Sensitivity to 5-Fluororacil Cytotoxicity

    doi: 10.3390/biology13100821

    Figure Lengend Snippet: JNK1/2 and p38 signaling affects CREB and STAT3 phosphorylation and subsequent 5-FU-initiated cytotoxicity in Visfatin-stimulated HCT-116 CRC cells. ( A ) HCT-116 CRC cells were pretreated with DMSO (vehicle), SP600125 (a JNK1/2 inhibitor at 20 μM), or SB203580 (a p38 inhibitor at 10 μM) for 30 min and then either maintained as controls or treated with Visfatin (50 ng/mL) for 4 h. The phosphorylation of CREB and STAT3 was determined using Western blot analysis. ( B ) HCT-116 CRC cells were pretreated with DMSO (vehicle), PD98059 (an ERK1/2 inhibitor at 25 μM), SP600125 (a JNK1/2 inhibitor at 20 μM), or SB203580 (a p38 inhibitor at 10 μM) for 30 min and then treated with vehicle (PBS) or Visfatin (50 ng/mL) for 1 h. Subsequently, the cells were then either maintained as controls or treated with 5-FU (10 μM) for 24 h. The viability of the treated cells was examined using the MTT assay. The results in ( A ) were obtained from three independent experiments, all of which exhibited similar outcomes. The data in ( A , B ) were calculated from the results of three independent experiments and are presented as the mean ± SEM. The results were considered statistically significant when p < 0.05. *, compared to untreated control cells. #, compared to cells treated with ( A ) DMSO and Visfatin and ( B ) 5-FU alone. ( B ) **, compared to cells treated with DMSO and 5-FU.

    Article Snippet: For antibody detection, the following primary antibodies were used, all procured from Cell Signaling Technology (Beverly, MA, USA): AREG (#42953, 1:800 dilution), phosphor-JNK1/2 (#4671, 1:1000 dilution), JNK1 (#3708, 1:1000 dilution), phosphor-p38 (#4511, 1:1000 dilution), p38 (#9212, 1:1000 dilution), phosphor-CREB (#9196, 1:500 dilution), CREB (#9197, 1:1000 dilution), phosphor-STAT3 (#9145, 1:1500 dilution), STAT3 (#9139, 1:1000 dilution), and β-actin (#3700, 1:2000 dilution).

    Techniques: Phospho-proteomics, Western Blot, MTT Assay, Control

    The effect of KG501 (a specific inhibitor p-CREB) on proliferation and apoptosis levels in OGD NSCs. (A) CART55-102(0.8 nM) treatment protects against loss of the structure of NSCs (Nestin, red) and enhances Nestin (Red) expression and KG501 inhibits the protection of structure in cultured oxygen and glucose deprived NSCs. Scale bar = 100 pm. (B) CART55-102(0.8 nM) treatment enhances Sox-2(Green) expression and KG501 inhibits the protection of neural stem cells in cultured oxygen and glucose deprived. Scale bar = 50 μm (C) CART55-102(0.8 nM) treatment increases the proliferation of oxygen and glucose deprived neural stem cells and KG-501 inhibits the proliferation of NSCs in cultured oxygen and glucose deprived. Scale bar = 100um. (D) Quantitative analysis of fluorescence results using ImageJ software. Data was presented as mean ± SEM, *** p < 0.001, ### p < 0.001. (E) The protein levels Bcl-2 and Bax were measured by Western blot in different groups. (F) Quantitative analysis of Bcl-2 expression. Blot images were cropped for comparison. All values are expressed as mean ± SEM of the band intensity of Bcl-2 normalized to Tubulin from 3 independent experiments. Compared with the OGD, after treatment CART55-102 (0.8 nM), the levels of Bcl-2 significantly increased. But the levels of Bcl-2 were significantly decreased after pre-treatment with KG501 in the CART treatment group. ** p < 0.001, *** p < 0.01, ## p < 0.01, ### p < 0.001. (G) Quantitative analysis of Bax expression. Blot images were cropped for comparison. All values are expressed as meant ± SEM of the band intensity of Bax normalized to Tubulin from 3 independent experiments. Compare with OGD, after treatment CART55-102(0.8 nM), the levels of Bax significantly decreased. But the levels of Bax were significantly increased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ### p < 0.001. (H) The protein levels of p-CREB were measured by Western blot in different groups. (I) Quantitative analysis p-CREB expression. Blot images were cropped for comparation. All values are expressed as mean ± SEM of the band intensity of p-CREB normalized to 3 independent experiments. Compare with the OGD, after treatment CART55-102(0.8 nM), the levels of p-CREB significantly increased. But the levels of p-CREB were significantly decreased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ## p < 0.01.

    Journal: Frontiers in Neuroscience

    Article Title: Cocaine amphetamine-regulated transcription peptide inhibits apoptosis in oxygen–glucose deprived neural stem cells

    doi: 10.3389/fnins.2024.1424719

    Figure Lengend Snippet: The effect of KG501 (a specific inhibitor p-CREB) on proliferation and apoptosis levels in OGD NSCs. (A) CART55-102(0.8 nM) treatment protects against loss of the structure of NSCs (Nestin, red) and enhances Nestin (Red) expression and KG501 inhibits the protection of structure in cultured oxygen and glucose deprived NSCs. Scale bar = 100 pm. (B) CART55-102(0.8 nM) treatment enhances Sox-2(Green) expression and KG501 inhibits the protection of neural stem cells in cultured oxygen and glucose deprived. Scale bar = 50 μm (C) CART55-102(0.8 nM) treatment increases the proliferation of oxygen and glucose deprived neural stem cells and KG-501 inhibits the proliferation of NSCs in cultured oxygen and glucose deprived. Scale bar = 100um. (D) Quantitative analysis of fluorescence results using ImageJ software. Data was presented as mean ± SEM, *** p < 0.001, ### p < 0.001. (E) The protein levels Bcl-2 and Bax were measured by Western blot in different groups. (F) Quantitative analysis of Bcl-2 expression. Blot images were cropped for comparison. All values are expressed as mean ± SEM of the band intensity of Bcl-2 normalized to Tubulin from 3 independent experiments. Compared with the OGD, after treatment CART55-102 (0.8 nM), the levels of Bcl-2 significantly increased. But the levels of Bcl-2 were significantly decreased after pre-treatment with KG501 in the CART treatment group. ** p < 0.001, *** p < 0.01, ## p < 0.01, ### p < 0.001. (G) Quantitative analysis of Bax expression. Blot images were cropped for comparison. All values are expressed as meant ± SEM of the band intensity of Bax normalized to Tubulin from 3 independent experiments. Compare with OGD, after treatment CART55-102(0.8 nM), the levels of Bax significantly decreased. But the levels of Bax were significantly increased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ### p < 0.001. (H) The protein levels of p-CREB were measured by Western blot in different groups. (I) Quantitative analysis p-CREB expression. Blot images were cropped for comparation. All values are expressed as mean ± SEM of the band intensity of p-CREB normalized to 3 independent experiments. Compare with the OGD, after treatment CART55-102(0.8 nM), the levels of p-CREB significantly increased. But the levels of p-CREB were significantly decreased after pre-treatment with KG501 in the CART treatment group. *** p < 0.001, ## p < 0.01.

    Article Snippet: Antibodies against Nestin, Sox, Synaptophysin CREB and phosphor-CREB (phosphor S133) were purchased from Abcam (Cambridge, MA, United States).

    Techniques: Expressing, Cell Culture, Fluorescence, Software, Western Blot, Comparison